Reporter

Part:BBa_J10063

Designed by: Malcolm Campbell   Group: iGEM2005   (2012-01-10)


Green Firefly Luciferase and LRE L. Cruciata (E. coli optimised)

The original part (K325229) was used as the template. We have removed all aspects of the pBad promoter and AraC regulatory DNA. This part now starts with the RBS in part K325229.

K325229 is based on part BBa_K325219. Two site-directed mutagenesis events have been carried out on the luciferase, an Asn286 Ser mutation was used to create part BBa_K325209 which is the green wild-type luciferase. Then a Val239Ile mutation was used to create a green bioluminescence.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2060
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 951
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 900
    Illegal AgeI site found at 2602
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 332
    Illegal SapI.rc site found at 2116


[edit]
Categories
Parameters
None